Ph.D Dietary Research

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Biography

I am a dietary researcher studying in Northeast England since 2017, whose topic is dietary interventions for cardiovascular health ageing. The research mainly focus on extra virgin olive oil (EVOO) consumption, which is a major component in the Mediterranean diet. Mediterranean diet is one of the high feasibility & acceptability diets for both Western & East Asian population. Mediterranean diet is also suggested to be the most effective diet for reducing cardiovascular health issues.

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My research aims to explore how EVOO intake changes cardiovascular risk factors, & effects of EVOO on biomarkers of inflammation and endothelial function on Caucasians and East Asians.  

Dietary Interventions for Cardiovascular Ageing

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Biological ageing is inevitable

A restricted lifespan, ageing and then death are basic characteristics of life. In nature, ...this is the basic problem of evolution.

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The unbalance between the paradox of life (oxygen, nutritional metabolities, biochemisstry process) and biological defenses (DNA repair...). Ageing is the shrinkage of the homeodynamic space.

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Cardiovascular health ageing improvement

The prevalence of healthy ageing is relatively low. Wide variation exists in the ageing among people is characterised by the degree to which body functions decline. Among the physiological and metabolic changes occurring with ageing, the ageing of heart function is the key determinant of health and disability.

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People become dissimilar from their contemporaries of the same chronological age as they become older due to lifestyle factors (75% contribution) mainly including dietary factors, and genetic factors (25% contribution). 

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Mediterranean diet & cardiovascular health

The Mediterranean diet has been widely recognized as a model of “healthy eating”. The traditional Mediterranean diet consists of a wide range of minimally processed whole grains and legumes, fresh vegetables, fresh fruits, cold pressed extra virgin olive oil, nuts, and seeds as the principal source of fat, moderate consumption of fish, low amounts of dairy products, very low frequency of intake of red and processed meat, and wine consumed in low to moderate amount.

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the Prevención con Dieta Mediterránea (PREDIMED) study

The PREDIMED (Prevención con Dieta Mediterránea) study is designed to assess the long-term effects of the Mediterranean diet (MeDiet) without any energy restriction on incident cardiovascular disease (CVD) as a multicenter, randomized, primary prevention trial in individuals at high risk. 

Key aspects for the dietary research

Plasma sample analysis

Plasma samples will be used for analysis of cardiovascular biomarkers using commercial Elisa kits according to the manufacturer’s specifications. Each Elisa plate was measured using a microplate spectrofluorometric reader (Tecan Spark®).

Human Trial 

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Research ethics

Trial deign & registeration

Primary outcome

Secondary outcome

Participants eligibility criteria and study settings

18-70y

male

Interventions
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Venepuncture & urine sample collect

BD Vacutainer® Safety-Lok™ blood collection set inserted into the antecubital vein in right arm; blood was drawn into separate 6 mL BD Vacutainer® Heparin Tube tubes (Becton Dickinson).

Blood pressure

Resting blood pressure

24-hour ambulatory blood pressure

Anthropometric & assessment of endothelial function by FMD, PWV

Body composition assessment

Handgrip strength

BMI

Basal MET.RATE  kcal

Dry lean weight kg (normal range: _____ - _____ kg)

etc...

Dietary assessment & nutritional analysis

‘‘Microdiet’’ software (Microdiet System, Version 6.4, Salford University, UK).

3-day food record & phone,messemgers, photos records

Outcome measures & data collected

Blood lipid

Established biomarkers: ELISA

Novel biomarkers: ELISA

Resting BP

ABP

Anthropometric measurements

Body composition assessment

Handgrip strength

Dietary assessment and nutritional analysis

   etc...

Sample size

G*Power (http://www.gpower.hhu.de/en.html) version 3.1.3 (Program written, concept and design by Franz, Universitat Kiel, Germany; freely available windows application software). 

Calculations considered a prior power of 0.8, a 0.05 significance level and a 0.5 correlation coefficient between groups. Sample size calculations were performed for the primary endpoint; this is, change in SBP response.

   etc...

Randomization and Allocation

Computer-generated random integer generator in blocks of 4, with a 1:1 ratio

etc...

Statistical analysis

Guidelines: CONSORT

Method: GLM etc...

Spices
Statistical analysis

IPAQ

Microdiet

SPSS

Comperhensive meta-analysis

Etc...

Outcomes

Data collection & summarize

Compare with research study

Results 
Example - ELISA
ELISA
ELISA
ELISA
ELISA
ELISA
ELISA
ELISA
ELISA
Example - microdiet
Micro 1
Micro 2
Micro 3
Example - NOA
NOA
NOA 2
NOA 3
NOA 4
NOA 5
Blood sample 1